Fig. 3

CircEPHA3 directly binds to miR-513a-3p in PCa cells. A CircBank, CircInteractome, and ENCORI have predicted twelve candidate circEPHA3 target miRNAs. B The schematic model displayed the preferred binding sites of circEPHA3 binding to 12 miRNA candidates. C Relative abundance of circEPHA3 in PC-3 and DU145 cells after RNA pull down. D Relative abundance of candidate miRNAs in PCa cells pulled down by biotinylated probes. E The percentage of input recovery of candidate miRNAs in PC-3 and DU145 cells pulled down by different probes. F The schematic of wild-type and mutant circEPHA3 luciferase reporter vectors. G Relative luciferase activity of HEK-293 T cells co-transfected with miR-513a-3p mimics or mimics NC, pmirGlo-wild type circEPHA3 (circEPHA3-wt) or pmirGlo-mutant type circEPHA3 (circEPHA3-mut) plasmids. H The FISH assay visualized the localization of circEPHA3 and miR-513a-3p in PCa cells. Scale bars = 10 μm. *, ** and *** represented P < 0.05, 0.01 and 0.001, respectively