Skip to main content
Fig. 7 | Journal of Translational Medicine

Fig. 7

From: Ubiquitin specific peptidase 37 and PCNA interaction promotes osteosarcoma pathogenesis by modulating replication fork progression

Fig. 7

Depletion of USP37 leads to alternation in replication fork and an increase in the number of stalled fork. A Examples of DNA fiber tract types representing ongoing Replication, stalled Replication fork, and new origins. B Representative images of DNA fiber tracts in control and USP37-depleted cells after HU treatment. CldU tracts were visualized in green and IdU was visualized in red in control and USP37-depleted cells. C Quantitative analysis of the DNA fiber replication restarts assay without HU treatment. Bar graphs show the quantification of total tracts with both CldU and IdU labels at the fork in control and USP37 depleted Osteosarcoma cells. The experiment was performed three times, and for each time point, about 300 fibers were counted. Error bars represent the standard deviation from three independent experiments. D Quantitative analysis of the DNA fiber replication restarts assay with HU treatment. Bar graphs show the quantification of total tracts with both CldU and IdU labels at the fork (CldU,IdU) in control and USP37-depleted Osteosarcoma cells. The experiment was performed three times, and for each time point, about 300 fibers were counted. Error bars represent the standard deviation from three independent experiments. E Quantitative analysis of the replication restart assay (Percentage of the Stalled fork) after HU treatment in cells transfected with si RNA control and si-RNA USP37

Back to article page