Fig. 1

The increased level of NRP-1 in cultured primary neurons after OGD injury and in rats after tMCAO injury. A Representative immunoblots and B Western Blot analysis of NRP-1 in the cultured primary cortical neurons after OGD; n = 4. Data are expressed as mean ± SEM. *p < 0.05, **p < 0.01, ***p < 0.001, as compared with the Cont group by one-way analysis of ANOVA with Bonferroni’s post hoc test. C Representative immunoblots and D Western Blot analysis of NRP-1 in the penumbra region after tMCAO; n = 4. Data are expressed as mean ± SEM. *p < 0.05, **p < 0.01, ***p < 0.001, as compared with the Sham group by one-way analysis of ANOVA with Bonferroni’s post hoc test. E Localization of immunoreactive NRP-1 expression on neurons before and after OGD. The left column displays neuronal marker class III-β-Tubulin (red) and nucleus DAPI (blue). The middle column shows expression of NRP-1 (green). The right column shows the co-localization of NRP-1, class III-β-Tubulin and DAPI. Scale bar: 20 μm. F Localization of immunoreactive NRP-1 expression on neurons before and after tMCAO. The left column displays neuronal marker NeuN (red). The middle column shows expression of NRP-1(green). The right column displays nucleus marker DAPI (blue). The last column shows the co-localization of NRP-1, NeuN and DAPI. Scale bar: 50 μm. (Cont = Control)