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Fig. 5 | Journal of Translational Medicine

Fig. 5

From: Disheveled3 enhanced EMT and cancer stem-like cells properties via Wnt/β-catenin/c-Myc/SOX2 pathway in colorectal cancer

Fig. 5

DVL3 promoted CSLCs and EMT phenotype of CRC via Wnt/β-catenin. A, B The effect of DVL3 on TOPflash and FOPflash luciferase activity were determined by a dual-luciferase reporter assay system in HCT-8 and SW620 cells. CD The expressions of CD44, CD133, E-cadherin, N-cadherin, DVL3 and β-catenin were detected by western blotting assay in HCT-8 and SW620 cells transfected with shNC, shβ-catenin, or shβ-catenin plus pcDNA3.1-DVL3. EF Above protein expressions were also determined by western blotting in HCT-8 and SW620 cells treated with shNC, shDVL3, or LiCL plus shDVL3 for 72 h. GH Transwell assay was used to test the potential migration and invasion of HCT-8 and SW620 cells treated with shNC, shDVL3, or LiCL plus shDVL3 for 72 h. I The ability of tumorsphere formation was examined in CRC cells treated with LiCL, LV-shDVL3, or LiCL plus LV-shDVL3. *P ≤ 0.05

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