Fig. 7
From: STIL/AURKA axis promotes cell proliferation by influencing primary cilia formation in bladder cancer
STIL interacted with AURKA and could maintain AURKA stabilization. A Co-Immunoprecipitation analysis shows that STIL can interact with AURKA. B, C Proteasome activity assay of STIL knockdown/overexpression and their corresponding control. D The western blot analysis for the protein expression (STIL and AURKA) in EJ cells was treated with different concentrations of MG132 (0, 1, 2, 4 μM). E The western blot analysis for the protein expression (STIL, AURKA, acetylated tubulin, and ARL13B) in siNC and siSTIL EJ cells treated with MG132 or not. F, G The western blot analysis for protein expression (STIL and AURKA) in STIL overexpression/STIL knockdown and their corresponding EJ cells treated with different concentrations of cycloheximide (CHX). All the percentages of protein levels were quantification. Unpaired t-test analysis was utilized to compare the differences between the two groups. H Schematic diagram of the STIL/AURKA/PC/SHH signalling in this study. *P-value < 0.05, **P-value < 0.01, ***P-value < 0.001, ****P-value < 0.0001