Fig. 6

STIL influences PC formation by regulating AURKA. A, B The protein expression levels (STIL, AURKA, acetylated tubulin, ARL13B, IFT88, SHH, GLI1, and SMO) in siNC and siSTIL EJ cells were transfected with siAURKA or not, respectively, were detected by western blot analysis. C, D The protein expression levels (STIL, AURKA, acetylated tubulin, ARL13B, IFT88, SHH, GLI1, and SMO) in control and STIL over-expressed EJ cells transfected with siAURKA or not, respectively, were detected by western blot analysis. All the percentages of protein levels were quantification. E, F siNC and siSTIL EJ cells were transfected with siAURKA or not, respectively, were serum-starved for 48 h, then fixed and stained with antibodies against ace-tubulin (red) and DAPI (blue) for immunofluorescence analysis. The scale bar indicates 10 μm. The percentage of ciliated cells was quantification. G, H Control and STIL overexpression EJ cells were transfected with siAURKA or not, respectively, were serum-starved for 48 h, then fixed and stained with antibodies against ace-tubulin (red) and DAPI (blue) for immunofluorescence analysis. The scale bar indicates 10 μm. The percentage of ciliated cells was quantification. I, J Control and STIL over-expressed EJ cells transfected with siNC or siAURKA were analyzed by wound-healing assay. The viability of control and STIL over-expressed EJ cells transfected with siNC or siAURKA, respectively, were analyzed by CCK8 assay. K The cell growth of control and STIL over-expressed EJ cells transfected with siNC or siAURKA were analyzed by clone formation assay. Unpaired t-test analysis was utilized to compare the differences between the two groups. *P-value < 0.05, **P-value < 0.01, ***P-value < 0.001, **** P-value < 0.0001