Fig. 5

AURKA is up-regulated in BLCA tissue and is highly correlated with STIL. A Biological progression enrichment analysis of four prognosis-related ciliary genes (STIL, AURKA, WDR62, and CENPF). B Venn diagram reveals the overlapping genes between DEGs of RNA-seq data and prognosis-related ciliary genes. C AURKA mRNA expression was downregulated in STIL knockdown EJ cells based on the RNA-seq analysis. D The Pearson correlation coefficient (PCC) showed the co-expression relationship between STIL and AURKA mRNA expression in the TCGA-BLCA datasets. E qRT-PCR assay reveals that AURKA was down-regulated by silencing STIL in bladder cancer cell. F Detection of the serum AURKA factor concentrations in the normal bladder population (Normal group, n = 40), patients with benign bladder disease (NC group, n = 10), and bladder cancer patients (BLCA group) (n = 40). G, H The AURKA expression data from GSE13507 was divided into high/low expression groups based on the median value. The Kaplan–Meier survival curves were depicted to show the cancer-specific survival (CCS) and overall survival (OS) in high/low AURKA expression. I The expression of AURKA protein in transfected and control cells was detected by western blot after transfection of EJ cells with lipofectamine 8000 reagent. J, K Western blot analysis for the protein expression (STIL, AURKA, IFT88, acetylated tubulin, ARL13B) in the AURKA-knockdown/AURKA overexpression groups. The percentage of protein levels was quantification. Unpaired t-test analysis was utilized to compare the differences between the two groups. *P-value < 0.05, **P-value < 0.01, ***P-value < 0.001, ****P-value < 0.0001