Fig. 2
From: STIL/AURKA axis promotes cell proliferation by influencing primary cilia formation in bladder cancer
STIL influences the proliferation, migration, cycle, and growth of BLCA cells. A, B The western blot analysis was performed to validate the STIL expression in STIL knockdown/overexpression compared to their corresponding control, respectively. The percentage of protein levels was quantification. C, D The migration ability for STIL knockdown/over-expression EJ cells compared to their corresponding control by the wound healing assays. E, F The viability of STIL knockdown/over-expression EJ cells compared to their corresponding control group was measured by CCK8 assay. G–I The cell growth for STIL knockdown/over-expression EJ cells compared to their corresponding control group by colony formation assays. The number of colonies was counted utilizing the software image J. J, K Cell cycle analysis of STIL knockdown/over-expression EJ cells compared to their corresponding control group. Unpaired t-test analysis was utilized to compare the differences between the two groups. *P-value < 0.05, **P-value < 0.01, ***P-value < 0.001, ****P-value < 0.0001