Fig. 1

STIL is a potentially essential PC-related protein in BLCA. A Analysis of the scale-free fit index and mean connectivity for various soft-thresholding powers in TCGA-BLCA datasets. B Checking the scale-free topology when β = 8. K represents the logarithm in the whole network connectivity, and p(k) shows the logarithm of the corresponding frequency distribution. The K value is negatively correlated with p(k). The correlation coefficient r2 was 0.91 in TCGA-BLCA datasets, representing scale-free topology. C The Dendrogram of all DEGs in TCGA-BLCA datasets clustered based on a dissimilarity measure (1-TOM). D The heatmaps were portrayed to represent the correlation between module eigengenes and clinical status (BLCA and normal status), and p-values and correlation coefficients were shown in every module. E Venn diagrams showed 7 overlapping genes between BLCA-related genes and cilia-related genes. F The Forest plot represents the hazard ratios of prognosis-related ciliary genes in TCGA-BLCA. The red point indicates the high hazard ratio. P-value < 0.05 is regarded as significant and identified by the red font. G Box plot showed the STIL mRNA expression in TCGA-BLCA and normal tissues. H The STIL expression data from TCGA-BLCA was divided into high/low expression groups based on the optimal cut-off value by x-tile software. The Kaplan–Meier survival curves were depicted to show disease-free survival (RFS) in high/low STIL expression groups. I STIL mRNA expression in normal bladder tissue, bladder cancer, and recurrence bladder cancer tissues from the GSE13507 dataset. J STIL mRNA expression in normal bladder mucosa, non-muscular infiltrating bladder cancer, and muscular infiltrating bladder cancer tissues from oncomine database. K Detection of the serum STIL factor concentrations in the normal bladder population (Normal group, n = 40), patients with benign bladder disease (NC group, n = 10), and bladder cancer patients (BLCA group) (n = 40). L, M The STIL expression data from GSE13507 was divided into high/low expression groups based on the median value. The Kaplan–Meier survival curves were depicted to show the cancer-specific survival (CCS) and overall survival (OS) in high/low STIL expression. Unpaired t-test analysis was utilized to compare the differences between the two groups. *P-value < 0.05, **P-value < 0.01, ***P-value < 0.001, ****P-value < 0.0001