Fig. 5

Tumor subtype1 cell evolutionary trajectory and functional enrichment analysis. A Epi T1-4 cell subsets were obtained by dimension reduction and clustering of tumor subtype1 subsets. B The cellular evolutionary trajectory of tumor subtype1: Time from 0 to 1, epithelial cell evolutionary trajectory direction from Epi T1-Epi T4, corresponding to global gene expression changes. Red is high expression and blue is low expression. C Marker genes of Epi T1-4 subsets. sigDown, downregulated genes; sigUp, upregulated genes. D Violin plots showing the expression of KRT5, VIM, SSTR2, LMP-1/BNLF2a/b, RPS18 and RPS23 genes in Epi T1-T4 subsets and the variation changes. E–H GSEA enrichment analysis of the respective marker genes of Epi T1-T4 subsets