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Fig. 3 | Journal of Translational Medicine

Fig. 3

From: Comprehensive single-cell transcriptomic and proteomic analysis reveals NK cell exhaustion and unique tumor cell evolutionary trajectory in non-keratinizing nasopharyngeal carcinoma

Fig. 3

NK cell subtype analysis and exhaustion landscape. A NK1-3 cell subsets were obtained by dimension reduction and clustering of NK cells. B Marker genes of NK1-3 subsets, sigDown: downregulated genes, sigUp: upregulated genes; The upregulated top5 genes in NK3 subset includes ZNF683. C GSEA results showed that nature killer cell-mediated cytotoxicity was activated in the NK1 subset; NES > 0, activated; NES < 0, inhibited. D GSEA results showed that nature killer cell- mediated cytotoxicity was inhibited in the NK2 subset. E GSEA results showed that cell adhesion molecules was activated in the NK3 subset. F Cell-cell interactions between mast cells and NK1-3 subsets. G Expression of cell exhaustion markers: HAVCR2, TIGIT, LAG3 and CTLA4 in NK1-3 subsets, with NK3 showing the highest expression of TIGIT and LAG3, and partial expression of HAVCR2 and CTLA4. Besides, NK3 showed the highest expression of ZNF683. H Representative images of immunohistochemical staining of TIGIT and LAG3 in the normal nasopharyngeal mucosa tissue and NK-NPC tissue. I NK cells in the GSE150825 dataset were downscaled and clustered into five cell subsets, nk1-5. J The expression of NKG7, ZNF683, TIGIT, HAVCR2, LAG3 and CTLA4 in the nk1-5 subsets, in which a relatively high expression of ZNF683, TIGIT and LAG3 was observed in the nk3 subset in NK-NPC. K The expression of TIGIT, LAG3, NKG7, HAVCR2 and ZNF683 were mapped to nk1-5 subsets, and TIGIT, LAG3 and ZNF683 were mainly expressed in the NK-NPC group, while they were less expressed in the NLH group

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