Fig. 7From: Protectin conjugates in tissue regeneration 1 alleviates sepsis-induced acute lung injury by inhibiting ferroptosisCREB mediates the effect of PCTR1 on eliminating lipid peroxides in vitro. 666-15 (CREB inhibitor, 1 μM) or an equivalent volume of DMSO was administered to H1299 cells for 30 min in advance, and then LPS and PCTR1 were co-administered for 48 h. A Immunofluorescence staining images of GPX4 (original magnification ×400). B, C Relative expression levels of GSH, MDA and 4-HNE. D The level of lipid peroxidation was determined with the C11-BODIPY 581/591 fluorescent probe (original magnification ×400). E Oxidized C11-BODIPY 581/591 probe was quantified by flow cytometry. Data are presented as the mean ± SD, n = 5–6. *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001 and ns: p > 0.05Back to article page