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Fig. 4 | Journal of Translational Medicine

Fig. 4

From: Protectin conjugates in tissue regeneration 1 alleviates sepsis-induced acute lung injury by inhibiting ferroptosis

Fig. 4

CREB mediates the effect of PCTR1 on eliminating lipid peroxides in LPS-induced ALI. PCTR1 at a dose of 200 ng was injected into each mouse via the caudal vein 6 h after LPS (15 mg/kg) administration. 666-15 (CREB inhibitor, 10 mg/kg) or an equivalent volume of DMSO was injected into the caudal vein 1 h before PCTR1 treatment. The mice were sacrificed 24 h after LPS stimulation. A–B Relative levels of MDA and 4-HNE. C Representative TEM images of each group. The black arrow indicates ferroptosis-like mitochondria. Magnification ×30,000. D Representative H&E staining of lung tissues (original magnification, ×200; inset, ×400). E Acute lung injury score. F The relative mRNA expression levels of the inflammatory cytokines: TNF-α, IL-1β and IL-6. The acute lung injury score data are presented as the median and range (25th–75th percentile), and other data are presented as the mean ± SD. n = 4–6. *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001 and ns: p > 0.05

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