Fig. 3

PCTR1 activates CREB via the ALX/PKA pathway to increase GPX4 expression in vivo. PCTR1 at a dose of 200 ng was injected into each mouse via the caudal vein 6 h after LPS (15 mg/kg, ip) administration. BOC‐2 (ALX receptor inhibitor, 600 ng/kg), H89 (PKA inhibitor, 10 mg/kg), 666-15 (CREB inhibitor, 10 mg/kg) or an equivalent volume of DMSO was injected into the caudal vein 1 h before PCTR1 treatment. The mice were sacrificed 24 h after LPS stimulation. A, B The protein expression level of P-PKA was determined by western blotting. C, D The protein expression level of P-CREB was determined by western blotting. E, F The protein expression level of GPX4 was determined by western blotting. Data are presented as the mean ± SD, n = 4–6. *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001 and ns: p > 0.05. ip: intraperitoneal