Fig. 5

MSI2 activity is negatively modulated by miR-143 in vitro (A) Key factors of Notch1 signaling pathway were determined in HEL and HL-60 cells transfected with miR-143 or miR-NC mimic. (B, C) Key factors of Notch1 signaling pathway (B) and tumor metastasis-associated proteins (C) were measured in Lenti- MiR-143 vector- or Lenti-Control vector-transfecting HEL cells. (D) Cell growth of MSI2-OE. HEL and NC. HEL cells transfected with miR-143 or miR-NC mimic was analyzed by CCK-8 assay. (E) Clonogenic capacity of Lenti-MSI2 vector- or Lenti-Control vector-transfecting HEL cells after the transfection with miR-143 or miR-NC mimic. Representative pictures (above) and statistical analysis diagram (below) were illustrated, scale bar = 200 μm. (F) Apoptosis in Lenti-MSI2 vector- or Lenti-Control vector-transfecting HEL cells transfected with miR-143 or miR-NC mimic. (G, H) Expression of MSI2, key factors of Notch1 signaling pathway and cancer stemness-related proteins (G) and tumor metastasis-associated proteins (H) were shown in Lenti-MSI2 vector- or Lenti-Control vector-transfecting HEL cells transfected with miR-143 or miR-NC mimic. Data are expressed as mean ± SD (error bars). * P < 0.05, ** P < 0.01 and ***P < 0.001, t-test