Fig. 1
MiR-143 is lowly expressed in AML and inhibits the development of AML. A Heatmap of miRNAs (Top 25 of upregulated miRNAs and Top 25 of downregulated miRNAs) in peripheral blood of newly diagnosed cytogenetically normal AML patients (AML; N = 12) and normal healthy donors (HD; N = 12) from a GEO Dataset (GSE142699). Hsa-miR-143-3p was marked with the red box. (B) Relative expression of miR-143 was determined in BM aspirates from healthy donors (N = 14) and newly diagnosed AML patients (N = 58). C Relative expression of miR-143 was further exhibited in different FAB subtypes. D AML patients were divided into the miR-143 high expression group (≥ 1.863e-4, N = 45) and low expression group (< 1.863e-4, N = 13) according to the optimal cutoff value (1.863e-4) for risk score and the OS of the patients was analyzed (P = 0.0073). (E) Comparison of cell proliferation in HEL cells transfected with miR-143 or miR-NC mimic by CCK-8 assay. F Colony formation in HEL cells decreased after the transfection of miR-143 mimic. Representative images of colonies (left panel) and statistical analysis diagrams of colony number and cell number in various AML cell lines and patient blast cells were shown (right panel), scale bar = 100 μm. (G) Cancer stemness-related proteins in HEL cells transfected with miR-143 or miR-NC mimic were determined by Western blotting. H Apoptosis of HEL cells and blasts from Patient#055 transfected with miR-143 or miR-NC mimic was assessed by Annexin V and PI staining. I Cell migration in EGFP-labeled HEL cells transfected with miR-143 or miR-NC mimic was detected by transwell assay and observed by fluorescence microscope, scale bar = 200 μm. J Migration of different AML cells was illustrated by counting cells from lower chambers. (K, L) BALB/c-Nu mice were subcutaneously transplanted with 3 × 106 MiR143-OE. HEL or NC. HEL cells to establish xenograft AML model. The tumor growth was measured via tumor volume, N = 4. Data are expressed as mean ± SD (error bars). * P < 0.05, ** P < 0.01 and ***P < 0.001, t-test