Fig. 8

Knockdown of HIGD2A attenuated the tumorigenicity of HCC cell lines in vivo. A–F Images of HepG2 (A), Huh7 (C) and MHCC97H (E) xenograft tumors in nude mice of shCtrl group and shHIGD2A.1 group (n = 5 mice per group), and comparison of the tumor weight between shCtrl group and shHIGD2A.1 group. Tumor volume changes of HepG2 (B), Huh7 (D) and MHCC97H (F) were monitored at indicated time points. G–I Immunohistochemical staining of ki67 in HepG2 (G), Huh7 (H) and MHCC97H (I) xenograft tumor tissues from shCtrl group and shHIGD2A.1 group, and the proportion of ki67 positive cells counted by ImageJ software. Results shown are mean ± SEM. A Mann–Whitney test was used. *P < 0.05 and **P < 0.01. Scale bar, 100 µm