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Fig. 7 | Journal of Translational Medicine

Fig. 7

From: HIGD2A silencing impairs hepatocellular carcinoma growth via inhibiting mitochondrial function and the MAPK/ERK pathway

Fig. 7

HIGD2A promotes stemness property of hepatocellular carcinoma. A Expression of genes associated with tumor stemness are significantly upregulated in patients with high HIGD2A mRNA expression. GSEA analysis of TCGA dataset (hepatocellular carcinoma). B Positive association of mRNAsi with HIGD2A expression. C, D The mRNA expression levels of the tumor stemness-associated markers (CD133, EpCAM, CD44, NANOG, ALDH1) in HepG2 (C) and Huh7 (D) transfected with shCtrl or shHIGD2A.1 were measured by real-time PCR. E Immunoblot analysis of the protein levels of CD133 and EpCAM in HepG2 and Huh7 transfected with shCtrl or shHIGD2A.1. Results shown are mean ± SEM. Pearson correlation was used in B and The Mann–Whitney test was used in C-D for statistical analyses. *P < 0.05

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