Fig. 3

HIGD2A depletion induces cell cycle S phase arrest in HCC cells. A, B Cell cycle analysis of HepG2 (A) and Huh7 (B) cells treated with shCtrl or shHIGD2A.1 analyzed by FACS, and the proportion of cell population in G1, S, and G2/M phases. C Left, the timeline of the treatment with aphidicolin. Right, HepG2 and Huh7 treated with shCtrl or shHIGD2A.1 were synchronized with aphidicolin for 24 h and analyzed by FACS for the cell cycle. D, E 18 h after the removal of aphidicolin, HepG2 (D) and Huh7 (E) were stained with PI and analyzed by FACS. Right, the proportion of cell population in G1/S and G2/M phases after washout of Aphidicolin. Results shown are mean ± SEM. The Mann–Whitney test was used for statistical analyses. *P < 0.05, **P < 0.01 and ***P < 0.001