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Fig. 2 | Journal of Translational Medicine

Fig. 2

From: HIGD2A silencing impairs hepatocellular carcinoma growth via inhibiting mitochondrial function and the MAPK/ERK pathway

Fig. 2

Silencing of HIGD2A impedes HCC cell proliferation and migration. A–C Western blotting assay for total HIGD2A protein expression following HIGD2A-knockdown in HepG2 (A), Huh7 (B) and L02 (C) cell lines. D, E Representative images of colony formation assay of HepG2, Huh7, and L02 cells transfected shCtrl or shHIGD2A.1 (D) and quantification of colony formation based on three independent assays (E). F The proliferation ability of HepG2, Huh7 and L02 cells infected with shCtrl or shHIGD2A.1 lentivirus was measured by CCK8 assay at the indicated time points. G The protein levels of caspase3 and cleaved-caspase3 were measured in HepG2, Huh7 and L02 cells infected with shCtrl or shHIGD2A.1 lentivirus. H, I Cell migration ability of HepG2, Huh7 and L02 cells transfected shCtrl or shHIGD2A.1 was analyzed by Transwell assay. Representative images of Transwell assay (H) and the quantitative data of migrated cells (I). Results shown are mean ± SEM. an unpaired t-test was used. *P < 0.05, **P < 0.01 and ***P < 0.001; ns, not significant. Scale bar, 100 µm

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