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Fig. 4 | Journal of Translational Medicine

Fig. 4

From: The activation of mTOR signalling modulates DNA methylation by enhancing DNMT1 translation in hepatocellular carcinoma

Fig. 4

mTOR hyperactivation upregulates the expression and activity of DNMT1. A Protein levels of DNMT1, S6K, P-S6K, P-4E-BP1 (T37/46), P-4E-BP1 (S65), P-4E-BP1 (T70), and 4E-BP1 determined by Western blot analysis in SNU423 and SNU449 cells stimulated with 100 ng/ml insulin for 12 h or 24 h. The values for P-S6K and P-4E-BP1 were normalized against the band intensities of S6K and 4E-BP1. B DNMT1 activity was detected in SNU423, SNU449 and MHCC-97H cells treated with DMSO and rapamycin (500 nM), as well as in WT and TSC1−/− MEFs, using a DNMT1 assay kit. The y-axis indicates DNMT1 activity as represented by the OD value. Data were presented as mean ± SD and each assay was performed for three times. C Linear correlation between p-mTOR IHC scores and DNMT1 IHC scores in tumoral regions of HCC patients. Correlations were analysed by Spearman’s rank correlation coefficient test. Ns, not significant, *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001

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