Fig. 5
The OSCC microenvironment is composed of heterogenous fibroblasts from which immunomodulatory cells are present across the metastatic cascade. A Overview of types of analyzed OSCC samples and their localization within the head and neck area. B Number of samples across patients colored by their respective tissue origin; for patient #4, the primary tumor could not be analyzed due to incorrect specimen processing; for patients #6 and #7 two regions of the primary tumor were analyzed, denoted as sample #6.1 and #6.4 and #7.1 and #7.4, respectively. C UMAP of 41,284 cells based on OSCC scRNAseq data from our cohort and colored by cell type. D UMAP of 21,037 cells based on CD45-negative and HPV-negative primary HNSCC from KĂĽrten et al. and colored by cell type. E UMAP of 1,595 fibroblasts and 551 pericytes from C colored by the respective phenotypes derived from shared-nearest neighbor clusters. F UMAP of 2,920 fibroblasts and 683 pericytes from D colored by the respective phenotypes derived from shared-nearest neighbor clusters