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Fig. 4 | Journal of Translational Medicine

Fig. 4

From: Mesenchymal–epithelial transition in lymph node metastases of oral squamous cell carcinoma is accompanied by ZEB1 expression

Fig. 4

ZEB1 is highly active in metastatic epithelial differentiated OSCC cells. A Percentage of tumor cells with detectable mRNA expression from scRNAseq (more than one UMI) encoding the indicted EMP-related transcription factors. B Mean inferred activity based on the target genes of the indicated transcription factors across tumor phenotypes from EMP-related patient-specific clusters. On top the log-normalized expression of CDH1 and cornifin-B (SPRR1B) is shown, on bottom the localization (primary tumor [PT] or lymph node metastasis [MET]) and respective EMP phenotype of the cluster. C Mean activity of ZEB1 for epithelial differentiated and pEMT clusters of each patient, respectively. Connecting lines show dots belonging to the same patient. ZEB1 (D) and cornifin-B (E) protein expression detected in serial sections by IHC of the primary tumor from patient #2; comparable areas are depicted. Scale bars equal 200 µm in overview and 100 µm in zoomed image. E Colocalized expression of ZEB1 (green) and cornifin-B (red) detected by double staining in the lymph node metastasis of patient #1. Nuclei are stained in blue (DAPI), Scale bars equal 10 µm

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