Fig. 5

RAB13 is overexpressed in OC and promotes OC cells migration and invasion. A Volcano plot revealed the DEGs between primary and metastatic OC cells (left) and the five genes with the greatest difference (right). B Intersection of the upregulated genes in metastatic OC cells and the hub genes of Epi7. C RAB13 and HES1 in the UMAP plot colored by expression level with pseudotime trajectory. D KM curve of overall survival time and RAB13 (top) or HES1 (bottom), n = 1656. E, F The mRNA expression levels of RAB13 were higher in OC cell lines than normal ovary cell line (*p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001 in t-test. N = 3), as well as the tissue protein levels based on the Human Protein Atlas (HPA) database. G, H Western blot analysis and qRT-PCR analysis of the expression of RAB13. The expression level of protein was quantified by grey analysis. (*p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001 in t-test. N = 3). I, J The wound healing assay and Transwell migration assay showed decreased migration ability of SK-OV-3 and A2780 cell lines treated with si-RAB13. (*p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001 in t-test. N = 3). K The Transwell invasion assay showed decreased invasion ability of SK-OV-3 and A2780 cell lines treated with si-RAB13. (*p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001 in t-test. N = 3)