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Fig. 3 | Journal of Translational Medicine

Fig. 3

From: Brevilin A is a potent anti-metastatic CRC agent that targets the VEGF-IL6-STAT3 axis in the HSCs-CRC interplay

Fig. 3

HSCs released IL6 that activated STAT3 in the CRC cells and increased cancer cells migration in the CRC-HSCs co-culture system. A, B Protein levels of p-STAT3 and STAT3 in HCT-116 (A), CT26 (B) cells and co-culture system were determined by using Western blotting (left panel); and quantitative results were analyzed using Image J software (right panel). C The IL6 content in the medium of HCT-116, HCT-116-LX-2 co-culture or IL6 neutralizing antibody-treated co-culture system were detected by using the ELISA assay. D Protein levels of p-STAT3 and STAT3 in HCT-116, HCT-116-LX-2 co-culture or IL6 neutralizing antibody-treated co-culture system were determined by using Western blotting (left panel); and quantitative results were analyzed using Image J software (right panel). E Representative photographs of cell migration in HCT-116, HCT-116-LX-2 co-culture or IL6 neutralizing antibody-treated co-culture system (left panel); and quantitative results were analyzed using Image J software (right panel). Data are shown as mean ± SD from three independent experiments. **P < 0.01, *P < 0.05 vs. the corresponding control. ##P < 0.01 vs. co-culture system. NAb: neutralizing antibody

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