Fig. 4

The tumor suppressive effect of CXCL12 on EC malignancy. A The expression of cytokines including CXCL12, IL8, HGF and TGF-βin human fibroblasts HS-27, TIG-1 and CRL-7815 induced by RB using WB assay. B The statistical analysis of WB assay. C The expression of cytokines including CXCL12, IL8, HGF and TGF-βin culture medium supernatant of human fibroblasts HS-27, TIG-1 and CRL-7815 induced by RB using ELISA assay. D, E Tumor proliferation ability of TE-1 and ECA-109 cells treated with 10 μg/mL CXCL12 using colony formation assay. F, G Tumor invasion ability of TE-1 and ECA-109 cells treated with 10 μg/mL CXCL12 using transwell assay. H, I Tumor apoptosis of TE-1 and ECA-109 cells treated with 10 μg/mL CXCL12 using flow cytometry assay. Data are presented as the mean ± standard error, n ≥ 3. ^*P < 0.05, ^**P < 0.01, ^***P < 0.001