Fig. 2

The responses of EC cells by human fibroblasts treated with 160 μg/mL RB in vitro. A, B Tumor proliferation ability of TE-1 and ECA-109 cells by human fibroblasts HS-27, TIG-1 and CRL-7815 treated with 160 μg/mL RB in vitro using colony formation assay. C, D Tumor invasion ability of TE-1 and ECA-109 cells by human fibroblasts HS-27, TIG-1 and CRL-7815 treated with 160 μg/mL RB in vitro using transwell assay. E, F Tumor apoptosis of TE-1 cells by human fibroblasts HS-27, TIG-1 and CRL-7815 treated with 160 μg/mL RB in vitro using flow cytometry assay. G, H Tumor apoptosis of ECA-109 cells by human fibroblasts HS-27, TIG-1 and CRL-7815 treated with 160 μg/mL RB in vitro using flow cytometry assay. All experiments are performed three times at least, and data are presented as the mean ± standard error. ^*P < 0.05, ^**P < 0.01, ^***P < 0.001