Fig. 2

CAFs impair the antitumor activity of CAR-T cells in vivo. a IHC staining of CAFs in 4T1 and E0771 breast and PANC02 and KPC1199 pancreatic tumor-bearing mice. b-f In vivo experimental of Antigen-Positive E0771 allografts. b Experimental scheme. Antigen-Positive E0771 tumor cells alone or combined with NIH 3T3 fibroblast were in situ inoculated into C57BL/6 mice, treated i.p. injection with CPA, and then given CAR-T cells (i.v.; n = 5 mice per group). c The tumor volume of tumors of each treatment group. d The tumor growth inhibition of each treatment group. e The tumor weight at the end point of the animal experiment. f CAR copy numbers in genomic DNA of residual tumors after therapy were measured by qRT-PCR (TaqMan probe). The images were obtained under original magnification 200×. Scale bars, 100 μm. All data are presented as the mean ± SEM of triplicate experiments. *p < 0.05, **p < 0.01, ***p < 0.001