Fig. 4

Quantitative RT-PCR of miRNA expression profiling data in validation cohort. qRT-PCR was conducted to measure the mRNA expression levels of let-7a, miR-122, miR-30d, miR-126, miR-27b, miR-4772 and miR-10a. The mean threshold cycle (Ct) was determined based on triplicate reactions. The 2^−ΔΔCt method was used to calculate the fold differences in EV-miRNA expression among the tested samples. miRNA expression was normalized against the spike-in cel-miR-39 as well as the endogenous miR-484 and miR-191. Data were reported as mean ± SEM. Significance was calculated with GraphPad Prism software by using the two-tailed t-test