Fig. 1From: Analysis of circulating extracellular vesicle derived microRNAs in breast cancer patients with obesity: a potential role for Let-7aCharacterization of extracellular vesicles (EVs) isolated from the serum samples of breast cancer patients with different BMI. A. Schematic illustration of the study design. EVs were purified from serum samples of Normal weight (NW; BMI < 25 kg/m2), and Overweight/Obese (OW/Ob; BMI ≥ 25 kg/m2) breast cancer patients by ExoQuick precipitation system. B. Representative images of transmission electron microscopy (TEM) showing EVs isolated from serum of NW and OW/Ob patients. Scale bar, 100 nm. C. Representative size distribution profiles and concentration of serum EVs measured by nanoparticle tracking analysis (NTA). D. Immunoblot analysis showing expression of the EV hallmarks Tsg101, CD9, CD63, CD81 and Alix in equal amount of EV lysates. The specificity of EV isolation was confirmed using and endoplasmic reticulum marker CalnexinBack to article page