Fig. 4

Effect of DAC treatment on the expression of stemness markers in the 1076 Col and 1872 Col CRC cell lines. RT-qPCR showed an up-regulation of mRNA level of Nanog (***p < 0.0001, ***p = 0.0008) (A), KLF4 (***p˂0.0001, ***p = 0.0005) (B), MSI1 (***p = 0.0003, ***p = 0.0004) (C), CD44 (***p = 0.0001, *p = 0.0120) (D), and CD133 (***p = 0.0003, *p = 0.0166) (E), respectively in both cell lines after DAC treatment. The results are represented as mean + SD from two independent experiments done in duplicates (Paired t-test)