Fig. 2

Effect of DAC treatment on autophagy markers. The 1076 Col and 1872 Col cells were treated with DAC (5 μΜ) for 48 h. and then analyzed by immunoblotting. Western blot analysis confirmed induction of autophagy via decreased Beclin-1 level (2.3 fold and 1.2 fold respectively) (a1, a2), increased LC3B protein level (1.67 fold and 1.14 fold respectively) (b1, b2), and decreased P62 level after treatment (twofold and 1.5 fold respectively) (c1, c2). β-actin served as the loading control. The band intensities were quantified using Image J and normalized against β-actin. The data are from duplicate experiments and presented as mean ± SD