Fig. 1

Effect of DAC treatment on cell proliferation and morphology in CRC cell lines, 1076 Col and 1872 Col. A Real-time cell proliferation (cell index) analysis of 1076 Col and 1872 Col CRC cells. Cells were grown in a monolayer on top of the electrodes and treated with 5 μΜ of DAC. The real-time cell analyzer was used to determine the cell index, as described in the methods section. B Trypan blue exclusion dye assay showed a significant decrease in growth in 1076 Col and 1872 Col CRC cells treated with 5 μΜ of DAC (twofold decrease and 2.5 fold decrease, respectively). C DAC treatment resulted in significant morphological changes in both cell lines, such as swelling, stretching, and intracellular particles. Images were captured using a bright-field microscope (Olympus IX51, objective 20×). The data shown are representative of at least three replicate experiments. Scale bar is 200 pixels