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Fig. 6 | Journal of Translational Medicine

Fig. 6

From: Exploring the putative role of PRDM1 and PRDM2 transcripts as mediators of T lymphocyte activation

Fig. 6

Gene expression analysis of lymphocyte activation related transcription factors in Jurkat transiently RIZ overexpressing cells. PRDM1 transcript levels were compared in Jurkat cells transiently transfected with the indicated encoding plasmids (with the expression value of the empty vector equal to 1). The ratio between the PR- and PR + transcripts for each gene was also calculated. RIZ2 overexpression reduced the ratio between BLIMP1a and BLIMP1b in favor of BLIMP1b. The expression of GATA3, TBX21, FOXP3, RORC, CD40LG was also measured in Jurkat cells in both basal conditions (ΔCt method as fold on RPS18 control gene) and after 36 h transient transfection with the plasmid encoding for RIZ1, RIZ2 or with pSG5 control (ΔΔCt method with the indicated control gene). Three independent experiments in triplicates were performed and data expressed as mean ± SD. ns (not significant), *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001.

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