Fig. 5

Gene expression analysis of PRDM1 and PRDM2 in Jurkat cells. On the left upper side, the basal levels of BLIMP1a, BLIMP1b, RIZ1, RIZex8 and RIZ2 in Jurkat cells are represented as fold on RPS18 control gene. The other bar graphs represent data from qRT-PCR analysis of the same transcripts in Jurkat cells following activation with PMA/Ion. Expression levels were calculated using the ΔΔCt method with the indicated control gene. Three independent experiments in triplicates were performed and data expressed as mean ± SD. ns (not significant), *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001