Fig. 1

The anti-fibrotic effect evaluation of microfat on human hypertrophic scars both in vivo and in vitro. A Histological staining of human hypertrophic scars which were transplanted to nude mice at four weeks after microfat injection. Black arrows indicate α-SMA-positive staining cells. B Number of α-SMA-positive myofibroblasts per high-power field. C The mRNA expression levels of fibrosis-related genes of human hypertrophic scars after microfat injection were detected by qRT-PCR. D Scratch images of HSFs alone (CTRL) and which co-cultured with microfat (CO) after 0 and 24 h. Red arrows draw the margin of scratch lines. E Migration rate of the HSFs in CTRL and CO groups. F CCK8 assay results of the HSFs in CTRL and CO groups. G The mRNA expression levels of fibrosis-related genes of HSF after co-cultured with microfat were detected by qRT-PCR. scale bar = 100 μm. Data are presented as the mean ± SD. *P < 0.05; **P < 0.01