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Fig. 2 | Journal of Translational Medicine

Fig. 2

From: Tumor-targeted superantigens produce curative tumor immunity with induction of memory and demonstrated antigen spreading

Fig. 2

C215Fab-SEA treatment induced a rapid T-cell influx into TDLNs and enhanced T-cell infiltration into the TME. A Violin plots showing the percentage of Vb3 CD4 (left) or CD8 (right) T cells of total CD45+ cells in the TDLNs as determined by FC. B Violin plots showing the cell density (cells/mg tumor tissue) of Vβ3 CD4+ (left) or CD8+ (right) T cells in tumors as determined by FC. C Violin plots showing the percentage of non-Vβ3 CD4 (left) or CD8 (right) T cells among total CD45 + cells in the TDLNs as determined by FC. D Non-Vβ3 CD8+/CD4+ T-cell ratios in TDLNs. E Violin plots showing the percentage of CCR7 + cells among non-Vβ3 CD8 T cells in the TDLNs. F Violin plots showing the cell density of non-Vβ3 CD4 (left) or CD8 (right) T cells in the TME. G Violin plots showing the CD8/CD4 T-cell ratios of non-Vβ3 cells found in the TME. AG FC analysis; n = 3–4/group/cycle; timepoints are referred to as C1-C4 (cycles 1–4, respectively). Statistical significance was determined by two-way ANOVA with Tukey’s multiple comparisons test. *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001, versus the timepoint-matched control; for C4 Combo versus C215Fab-SEA H Graphs displaying the mean (± SEM) log2-fold-change of immune populations according to their gene signatures (Additional file 5: Table S1) at each treatment cycle compared to a matched control group in tumors. n = 3–4/group/cycle. Statistical significance was determined by nSolver software I. Representative immunohistochemical (IHC) CD3 staining analysis of frozen sections (n = 2–3/group/cycle) of MC38-hEpCAM tumors from the control and treatment groups 24 h after the completion of cycle 3. Scale bar, 100 µm; IHC score (upper right): 1 +  = few positive cells, 1–2 +  = few to moderate numbers, 2 +  = moderate numbers, 2–3 + moderate to high numbers, 3 + high numbers. J Heatmap displaying the relative (mean log2-fold-change) expression of selected chemokine and adhesion genes associated with T-cell infiltration and chemotaxis compared to a matched control group in the TME (left). n = 3–4/group/cycle. The color gradient indicates the fold-change over the matched control. The right heatmap shows the p values of the significant differences in the left heatmap. White represents a nonsignificant expression change compared to the matched control. Differential expression analysis was performed with Welch’s t test

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