Fig. 5

Systemic administration of polarized CAR-Ms induced efficient antitumor effects in a B16F10 melanoma model. A An illustration of the experimental design. B Representative fluorescent images of melanoma tissue stained with anti-GFP to detect the infiltration of GFP-positive anti-HER2 CAR-Ms, scale bar: 100 μm. C Tumor growth of C57 mice bearing subcutaneous B16F10-HER2 melanoma tumors in the GFP-M (black), GFP-M1 (green), CAR-M (blue), and CAR-M1 (red) treatment groups. The average tumor volume per treatment group is presented as the mean ± S.D. D Kaplan‒Meier survival curve for (C) mice bearing B16F10-HER2 cells after receiving GFP-Ms, GFP-M1s, CAR-Ms, and CAR-M1s (n = 6 mice/group). E Body weight changes in tumor-bearing mice posttreatment. The data are shown as the mean ± S.D. F Tumor growth of individual mice from (C). Each line represents an individual mouse. *, p < 0.05; **, p < 0.01; ***, p < 0.001