Fig. 8

High lactate is the key factor for IL-8 to promote Tregs infiltration in vivo. A. Quantification of Tregs (FOXP3 + , red arrow) and CD8 + T cell infiltration in the four mouse orthotopic HCC groups; immunohistochemistry was used to locate the Tregs and CD8 + T cells; scale bar, 50 µm. B. The infiltration level of Tregs and CD8 + T cells in HCC tissue of the four mice orthotopic HCC groups (n ≥ 3); Three visual fields were randomly selected for cell counting in each sample. The data represent the mean ± S.D. Mann–Whitney U test or t–tests were used for statistical analysis. *P < 0.05; **P < 0.01; ***P < 0.001. Abbreviations: KD, knockdown; OE, overexpression