Fig. 7
IL-8 combined with high lactate promotes the development of HCC. A. Quantification of IL-8 expression in the four groups including DAPK1 OE, IL-8 OE, IL-8 OE with DAPK1 OE, and control Hepa1-6 groups; the number of cells is 1 × 105; determination after 24 h of culture. B. Quantification of lactate production in four groups of Hepa1-6 cells; determination after 24 h of culture. C. The protein expression of DAPK1 in four groups of Hepa1-6 cells. D. The expression of IL-8 and DAPK1 in the four mice orthotopic HCC groups; Immunohistochemistry was used to determine IL-8 and DAPK1 protein expressions; Scale bar, 50 µm. E. Quantification of lactate concentration in tumor tissues of the four mouse orthotopic HCC groups (n ≥ 3). F. In vivo fluorescence images of the four mouse orthotopic HCC groups (n = 5); Three weeks after Hepa1-6 implantation. G. Macroscopic tumor growth in the four groups orthotopic HCC model. The data represent the mean ± S.D. Mann–Whitney U test or t–tests were used for statistical analysis. *P < 0.05; **P < 0.01; ***P < 0.001. Abbreviations: KD, knockdown; OE, overexpression