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Fig. 3 | Journal of Translational Medicine

Fig. 3

From: Tumor cell density dependent IL-8 secretion induces the fluctuation of tregs/CD8 + T cells infiltration in hepatocellular carcinoma: one prompt for the existence of density checkpoint

Fig. 3

The fluctuation of IL-8 expression on the tumor cell density axis is consistent with Tregs/CD8 + T cell infiltration. A. Quantification of tumor cell density in HCC dataset of TCGA database; Five visual fields were randomly selected for cell counting in each sample. B. Ratio of infiltration degree of Treg and CD8 + T cell after grouping by HCC cell density (< 5000 cells/mm2, 5000–6000 cells/mm2 and ≥ 6000cells/mm2); Data obtained from the TCGA database; Quantification of infiltration degree based on CIBERSORT-ABS algorithm. C. Trends of FOXP3 + /CD8 + ratio with tumor cell density and grouping. D. mRNA expression of soluble factors in HCC after grouping by HCC cell density (< 5000 cells/mm2, 5000–6000 cells/mm2 and ≥ 6000cells/mm2); The number shows the expression of factors with significant difference. Data obtained from the TCGA database. E. The protein expression of IL-8 in HCC after grouping by HCC cell density (< 5000 cells/mm2, 5000–6000 cells/mm2 and ≥ 6000cells/mm2); Scale bar, 50 µm. F. Quantification of IL-8 protein expression in HCC after grouping by HCC cell density (< 5000 cells/mm2, 5000–6000 cells/mm2 and ≥ 6000cells/mm2) (n = 65); Image J was used for quantification. The data represent the mean ± S.D. ANOVA or Kruskal–Wallis test were used for statistical analysis. *P < 0.05; **P < 0.01; ***P < 0.001

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