Fig. 1
The change trend of FOXP3 + , CD8 + , CD206 + and CD86 + cells with tumor cell density. A. Counting tumor cell density and immune cell density in the same visual field. B. Quantification of tumor cell density (n = 65); Five visual fields were randomly selected for cell counting in each sample. C. Quantification of CD8 + and FOXP3 + cells density in HCC by immunohistochemistry (n = 65); Five visual fields were randomly selected for cell counting in each sample. D. Trends of FOXP3 + /CD8 + ratio with tumor cell density and grouping. E. FOXP3 + and CD8 + infiltration degree after grouping by HCC cell density (< 5000 cells/mm2, 5000–6000 cells/mm2 and ≥ 6000cells/mm2); Scale bar, 50 µm. F. Quantification of CD206 + and CD86 + cells density in HCC by immunohistochemistry (n = 65); Five visual fields were randomly selected for cell counting in each sample. G. Trends of CD206 + /CD86 + ratio with tumor cell density and grouping. H. CD206 + and CD86 + infiltration degree after grouping by HCC cell density (< 5000 cells/mm2, 5000–6000 cells/mm2 and ≥ 6000cells/mm2); Scale bar, 50 µm. I. Comparison of FOXP3 + /CD8 + ratio and CD206 + /CD86 + ratio between < 5000 cells/mm2 (n = 23), 5000–6000 cells/mm2 (n = 14) and ≥ 6000cells/mm2 (n = 28). The data represent the mean ± S.D. ANOVA or Kruskal–Wallis test were used for statistical analysis. *P < 0.05; **P < 0.01; ***P < 0.001