Fig. 4

Experimental verification the expression of OAS gene family in HF. A Representative heart sizes from each group (left of upper row) and M-mode echocardiography of heart (right of upper row), measurement of HW/BW, EF%, FS%, LVPWD, and LVPWS, n = 4 samples for each group. B qPCR analysis of ANP, BNP, β-MHC, OAS1, OAS2, OAS3 and OASL mRNA levels in sham and 8W TAC hearts. Data were normalized to the GAPDH expression, n = 4 samples for each group. ANP, BNP, and β-MHC are HF markers. C H9C2 cells treated with PBS and Ang II (1 μM) for 48 h and performed to determine cell size with phalloidin and DAPI, n = 3 samples for each group. D qPCR analysis of ANP, BNP, β-MHC, OAS1, OAS2, OAS3 and OASL mRNA levels in PBS and 1 μM Ang II-treated H9C2 cells. Data were normalized to the GAPDH expression, n = 3 samples for each group. ANP, BNP, and β-MHC are HF markers. * P < 0.05, ** P < 0.01, *** P < 0.001