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Fig. 6 | Journal of Translational Medicine

Fig. 6

From: LncRNA H19-rich extracellular vesicles derived from gastric cancer stem cells facilitate tumorigenicity and metastasis via mediating intratumor communication network

Fig. 6

H19 delivered by CSCs-EVs facilitates the malignant behaviors of NSCCs by disrupting the YAP/CDX2 signaling axis. A mRNA expression of CDX2 in NSCCs treated with oe-CDX2 detected by RT-qPCR. B Western blot of CDX2 protein in NSCCs treated with oe-CDX2. C H19 expression and CDX2 mRNA expression in NSCCs analyzed by RT-qPCR. D Western blots and quantification analysis of the total YAP and nuclear YAP proteins and CDX2 protein in the NSCCs co-cultured with CSCs-EVs-sh-H19 + oe-NC or CSCs-EVs-sh-H19 + oe-CDX2. The grayscale analysis of the relative nuclear YAP expression used H3 as the internal reference, and that of the total YAP protein expression and CDX2 protein expression used β-actin as the internal reference. E Viability of NSCCs co-cultured with CSCs-EVs-sh-H19 + oe-NC or CSCs-EVs-sh-H19 + oe-CDX2 detected by CCK-8 assay. F Migration and invasion of NSCCs co-cultured with CSCs-EVs-sh-H19 + oe-NC or CSCs-EVs-sh-H19 + oe-CDX2 detected by Transwell assay. G Western blot of the stemness marker proteins CD44 and CD133 in the NSCCs co-cultured with CSCs-EVs-sh-H19 + oe-NC or CSCs-EVs-sh-H19 + oe-CDX2. Cell experiments were conducted three times independently. *p < 0.05, **p < 0.01

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