Fig. 5

H19 delivered by CSCs-EVs induces YAP activation and augments CDX2 expression in NSCCs. A Expression of active YAP in the nucleus of NSCCs co-cultured with CSCs-EVs or CSCs-EVs-sh-H19 detected by immunofluorescence staining. B Expression of YAP and CDX2 in the nucleus of NSCCs treated with Verteporfin detected by immunofluorescence staining. C mRNA expression of CDX2 in NSCCs treated with Verteporfin determined by RT-qPCR. D H19 and CDX2 expression in NSCCs treated with CSCs-EVs + DMSO or CSCs-EVs + Verteporfin detected by RT-qPCR. E Expression of active YAP and CDX2 in the nucleus of NSCCs treated with CSCs-EVs + DMSO or CSCs-EVs + Verteporfin detected by immunofluorescence staining. F Western blots and quantification analysis of nuclear YAP, total YAP and CDX2 proteins in NSCCs treated with CSCs-EVs + DMSO or CSCs-EVs + Verteporfin. The grayscale analysis of the relative nuclear YAP expression used H3 as the internal reference, and that of the total YAP protein expression and CDX2 protein expression used β-actin as the internal reference. Cell experiments were conducted three times independently. *p < 0.05, **p < 0.01