Fig. 2From: LncRNA H19-rich extracellular vesicles derived from gastric cancer stem cells facilitate tumorigenicity and metastasis via mediating intratumor communication networkCSCs-EVs elevate the malignant behaviors of NSCCs and stemness marker protein expression. A Morphology of CSCs-EVs observed under a TEM. B NTA of the particle size of CSCs-EVs. C Western blot of the EV positive markers CD81, CD9, Alix, and the negative markers HSP90 and histone H3 in CSCs-EVs. D Immunofluorescence staining of the uptake of CSCs-EVs by NSCCs. E Viability of NSCCs co-cultured with CSCs-EVs measured by CCK-8 assay. F Migration and invasion of NSCCs co-cultured with CSCs-EVs measured by Transwell assay. G Western blot of the stemness marker proteins CD44, CD24, CD133, and Lgr5 in NSCCs co-cultured with CSCs-EVs. Cell experiments were conducted three times independently. *p < 0.05Back to article page