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Fig. 1 | Journal of Translational Medicine

Fig. 1

From: LncRNA H19-rich extracellular vesicles derived from gastric cancer stem cells facilitate tumorigenicity and metastasis via mediating intratumor communication network

Fig. 1

Isolation and identification of CSCs and NSCCs. A The expression of stem cell surface markers CD44 and CD24 in human GC cell lines detected by flow cytometry. The II and III quadrants indicate CD44+, and the III quadrant indicates CD44+ and CD24+. B Colony formation of GC cell lines in serum-free medium. C Western blots and quantification analysis of the stemness marker proteins CD44, CD24, CD133, and Lgr5 in the tumorsphere and adherent cells of each GC cell line. TCs indicate tumorsphere cells and ACs indicate adherent cells. D Sphere-forming ability of CD44+CD24+ and CD44−CD24− cells in HGC-27 cells. E Tumor volume of mice inoculated with CSCs (CD44+CD24+) and NSCCs (CD44−CD24−) at 1–7 weeks. F Tumor weight of mice inoculated with CSCs (CD44+CD24+) and NSCCs (CD44−CD24−). Cell experiments were conducted three times independently. n = 8 mice for each treatment. *p < 0.05

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