Fig. 2

Longitudinal variations of enterotype in each patient with good or poor response to nCCRT. a, Nonmetric multidimensional scaling analysis of amplicon sequencing variants (ASVs) of all samples, indicating that all samples could be assigned to one of three community state types (CSTs). b Relative abundance of the top 19 most abundant genus-level microbes in the three CSTs. c Bacterial CSTs over time in individual patients before, during and after nCCRT. Patients were categorized in terms of response (good vs poor). d Overview of CST proportions in patients with good response to nCCRT (top) and poor response to nCCRT (bottom). *P < 0.05. No one in the good-response group displayed CST1 type during nCCRT. e Linear discriminant analysis effect size cladogram displaying the taxa enriched in CST1 (green), CST2 (yellow), and CST3 (purple). Taxa enriched within higher-level taxa are shown by darker shading. f Bacteria (at the genus level) showing the greatest changes in abundance in each CST