Fig. 7

Nr2f6 directly binds to the promoter of Grpel2 and regulates Grpel2 expression. A Venn diagram showing the candidate transcription factors identified in the JASPAR and GENECARDS databases. B Scatter plots showing the Pearson correlations for the mRNA levels of Grpel2 and candidate transcription factors in human left ventricular samples from the GTEx database through the GEPIA website. C Scatter plots showing Pearson correlation analysis of mRNA levels of Nr2f6 and Grpel2 in control or diabetic heart tissue based on the GEO database (GSE123975). D, E Western blotting and quantitative analysis of heart Nr2f6 protein expression in the heart tissues of mice at 0, 6 and 12 weeks after STZ injury (n = 4/group). F qRT-PCR of Grpel2 mRNA levels in heart tissues of mice at 0, 6 and 12 weeks after STZ injury (n = 4/group). G Chromatin immunoprecipitation (ChIP) and qRT-PCR analysis of the binding of Nr2f6 to the Grpel2 promoter in NCMs (n = 4/group). H, I Western blotting and quantitative analysis of Nr2f6, mitochondrial Grpel2 and mitochondrial DLST protein expression in NCMs infected with siCtrl or siNr2f6 under NG or HG conditions (n = 4/group). J, K Western blotting and quantitative analysis of Nr2f6, and mitochondrial Grpel2 protein expression in NCMs infected with Ad-EV or Ad-Nr2f6 under NG or HG conditions (n = 4/group). Data are presented as the mean ± SD. Data in B, C were analysed by Pearson correlation analysis. Data in I, K were analysed by one-way ANOVA, followed by Tukey’s post hoc test. Other data were analysed by an unpaired, 2-tailed Student’s t test. *p < 0.05; ns, not significant