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Fig. 5 | Journal of Translational Medicine

Fig. 5

From: Grpel2 maintains cardiomyocyte survival in diabetic cardiomyopathy through DLST-mediated mitochondrial dysfunction: a proof-of-concept study

Fig. 5

Grpel2 promotes the process of DLST import into mitochondria. A Co-immunoprecipitation (IP) analysis of DLST with Grpel2 in whole-cell lysates of NCMs. B Co-IP analysis of Grpel2 with DLST in whole cell lysates of NCMs. C, D Western blotting and quantitative analysis of Grpel2 and DLST protein expression in NCMs infected with siCtrl or siGrpel2 under NG conditions (n = 4/group). E, F Western blotting and quantitative analysis of Grpel2 and DLST protein expression in the whole cell, cytoplasm or mitochondrial lysates of NCMs infected with Ad-EV or Ad-Grpel2 under NG or HG conditions (n = 4/group). G, H Western blotting and quantitative analysis of Grpel2 and DLST protein expression in the whole cell, cytoplasm or mitochondrial lysates of NCMs infected with siCtrl or siGrpel2 under HG conditions. Data are presented as the mean ± SD. Data in H were analysed by an unpaired, 2-tailed Student’s t test. Other data were analysed by one-way ANOVA, followed by Tukey’s post hoc test. *p < 0.05; ns, not significant

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